Southern two-striped walkingstick possesses a defense mechanism whereby it secretes a chemical upon touch that is non-toxic to mildly toxic to humans. This iridoid compound can cause mild skin irritation and should symptoms occur, they are typically short-lived. However, concentrated exposure may lead to more severe irritation or discomfort. No long-term effects are expected from casual contact with southern two-striped walkingstick.

Southern two-striped walkingstick poses a unique threat as its adults are capable of spraying a chemical toxin that can affect a range of animals. This spray can cause mild to severe effects. For instance, humans may experience intense burning sensations or temporary blindness if the toxin contacts the eyes, while pets like dogs and cats may exhibit signs of discomfort and pain. Smaller mammals could also suffer from the potency of this toxin. Even insects, despite their diverse physiologies, can be deterred or harmed by southern two-striped walkingstick's defensive spray.

Southern two-striped walkingstick has developed a defense strategy that involves spraying a chemical substance when it feels threatened. The secretion of this irritant is a survival mechanism to deter predators. Unfortunately, humans come into contact with this defense mechanism when they handle or disturb southern two-striped walkingstick, leading to possible skin irritation or discomfort.

To minimize issues with southern two-striped walkingstick, observe them without physical contact. Educate those around you, especially children, on the risks of handling or disturbing this insect. Habitat modification, such as clearing away brush or debris where they may reside, can reduce encounters. Use protective gloves if handling is necessary.

If you experience irritation from southern two-striped walkingstick, wash the affected skin with soap and water promptly to remove any irritants. Avoid rubbing your eyes or touching your face after contact. Rest in a well-ventilated area and refrain from unnecessary physical agitation of the affected skin to reduce symptoms. During recovery, continue to keep the skin clean and monitor for any signs of increased irritation, seeking professional advice if symptoms persist.

As a result of southern two-striped walkingstick's feeding habits, it chews on the tissues of leaves and stems. This leads to physical damage that can be observed through bite marks and missing plant material. The structural integrity of the plant is compromised, which may disrupt the plant's normal growth and photosynthesis processes. This damage could belie reduced vigor and overall health of the host plant.