Hasselt's spiny spider has a mild toxin where both juveniles and adults use their fangs to deliver venom when biting. This may cause discomfort or mild reactions in animals such as humans, dogs, cats, and birds. Lizards and insects might also be affected, experiencing localized effects from the venom. The organism typically uses its toxin for defense and predation, and the varied reactions among different animals depend on their respective physiological sensitivity to the toxin.

Hasselt's spiny spider is not known to exhibit behavior that involves biting humans. Generally, they may bite if threatened, but this species typically does not actively seek to bite humans, and such occurrences are rare and mainly defensive rather than offensive.

Hasselt's spiny spider can occasionally cause distress to humans, primarily due to their web-building in places frequented by people. While hasselt's spiny spider rarely bites, their webs can be a nuisance and may lead to accidental entanglement, which can cause discomfort and unease, especially for those with arachnophobia.

To prevent issues associated with hasselt's spiny spider, regular cleaning and removal of webs in and around the home are effective. Use of natural repellents such as eucalyptus, peppermint oil, or citrus sprays in areas where hasselt's spiny spider is common can deter them from establishing webs. Maintaining a tidy environment reduces the likelihood of hasselt's spiny spider inhabiting human spaces.

If experiencing discomfort from accidental contact with hasselt's spiny spider, calmly remove any web remnants. Ensure that living spaces are well-maintained to prevent further incidents. Use of non-toxic, natural deterrents in corners and less-disturbed areas can discourage hasselt's spiny spider presence. During recovery from distress, it's important to avoid disturbing hasselt's spiny spider habitats, as they are more prone to defensive behavior when threatened.