Southern house spider typically poisons its prey or potential threats through bites with its fangs. This species has a venom of mild intensity, which can cause discomfort or mild reactions in animals such as humans, dogs, and rats. Smaller creatures, like flies and bees, might suffer more severe consequences due to their size and physiology. The adults of southern house spider are primarily responsible for this toxin-related defense and hunting mechanism.

Southern house spider typically does not bite humans as it is not characteristic of this species; they are often reluctant to bite unless threatened or provoked. When bites occur, they involve a quick bite as a defensive method but generally result in mild consequences for humans.

Southern house spider seldom interacts with humans, but when they do, it is usually because their habitat has been disturbed. As reclusive creatures, they prefer undisturbed, dark recesses. Troubles with humans arise mainly when cleaning or moving objects under which southern house spider may reside, prompting a defensive response if they feel threatened.

To prevent encounters with southern house spider, maintain a clean and clutter-free environment. Regularly inspect dark, undisturbed areas such as garages, attics, or basements and clear away any webbing. Utilizing sealants to close off entry points and reduce harborage options can also deter southern house spider from making a home near human dwellings.

If troubles from southern house spider arise, calmly and carefully remove the arachnid from the human vicinity using non-harmful methods such as guiding it with a broom onto a piece of paper or into a container for release outside. During this process, ensure to wear gloves to limit direct contact. Afterward, thoroughly clean the affected area, while also employing regular maintenance to reduce further incidents. During recovery, avoiding disturbance of similar areas is key until ensuring they are free of southern house spider.