Lycosa singoriensis's venom contains neurotoxins that can induce mild symptoms in humans, such as localized pain, redness, and swelling. Adults use strong fangs to deliver venom primarily for prey immobilization and secondarily in defense. Bites to humans are rare and generally not medically significant, with no severe long-term effects. The risk to human health remains low.

Lycosa singoriensis typically has a mild level of toxicity, which primarily affects animals through stinging. This organism's sting may cause discomfort to humans and pets such as dogs and cats, while reactions can vary among different individuals. Generally, the sting might result in localized pain, swelling, and redness. Some animals may experience more intense reactions, but such occurrences are less common, and severe complications are rare with lycosa singoriensis's venom.

Lycosa singoriensis, typically does not bite humans as it is not characteristic of their behavior. If such an event occurs, it is likely defensive, involving a simple bite mechanism with mild consequences.

Lycosa singoriensis is principally a hunter that might inadvertently wander into human habitation in search of prey. As mostly terrestrial creatures, they come into contact with humans when their outdoor habitats overlap with areas of human activity. Troubles often arise from unintentional interactions, such as when a human unknowingly encroaches on lycosa singoriensis's space, provoking a defensive response.

To prevent negative encounters with lycosa singoriensis, seal cracks and gaps in homes to deter entry. Remove clutter and debris near dwellings to eliminate their hiding spots. Employ physical barriers like screens on windows and vents. Use LED lights that attract fewer insects outside, thus reducing the food source that might lure lycosa singoriensis closer to human habitats.

In the instance of a defensive interaction with lycosa singoriensis, thoroughly cleanse the affected area with soap and water to lessen the risk of infection. Apply a cold compress to mitigate swelling and discomfort. To minimize adverse consequences, avoid disturbing their natural habitats during outdoor activities and exercise caution when moving objects under which lycosa singoriensis might reside. During recovery, refrain from scratching or irritating the affected site, ensuring a swift and less troublesome healing process.