Australian golden orb-weaver is a mild toxin producer, which typically uses its fangs to inject venom into prey or in defense. This species' venom has a rather limited effect, primarily causing discomfort or mild reactions in animals such as humans, dogs, cats, and various garden birds. Among smaller creatures like frogs, flies, and beetles, the effects might be more pronounced, potentially leading to their immobilization or demise. Typically, australian golden orb-weaver poses little threat to larger animals, and serious problems from its bite are rare.

Australian golden orb-weaver rarely bites humans, with such events typically occurring when adults are provoked or defensively reacting. Bites are mild and the immediate effects are minimal, largely due to australian golden orb-weaver's non-aggressive nature towards humans.

Australian golden orb-weaver rarely interacts with humans; however, issues arise when their habitats overlap with human dwellings or outdoor activities. Troubles can occur when australian golden orb-weaver, feeling provoked or threatened, defensively bites a human. While typically non-aggressive, their presence can be alarming and bites, though generally mild, may cause discomfort.

Preventative measures focus on minimizing habitat overlap. Regularly clearing and maintaining garden areas can dissuade australian golden orb-weaver from nesting. Use of physical barriers like window screens and keeping living spaces tidy reduces the chances of indoor encounters. Educate residents on the importance of not disturbing australian golden orb-weaver webs and habitats.

In the event of a bite, it is important to remain calm and keep the bitten area clean. Washing with soap and water can prevent infection. Cold compresses may alleviate discomfort or any mild swelling. It's advisable to avoid disturbing the bite site further. During recovery, monitoring the bite for changes and maintaining cleanliness is crucial. Reassurance that australian golden orb-weaver is typically non-harmful can be comforting.