The venom of eastern grass orb-weaver is utilized primarily for predation and defense. It secretes venom through its fangs to incapacitate prey and deter potential threats. While typically mild to most animals, the venom can cause discomfort and localized reactions. Humans, dogs, and cats might experience pain or slight swelling from a bite, whereas small birds and lizards may face more serious paralysis or life-threatening effects due to their size and physiological differences. Adult eastern grass orb-weaver are the life stage when they are capable of envenoming.

Eastern grass orb-weaver does not typically exhibit biting behavior towards humans as it is not characteristic of this species. Instances of humans being bitten by eastern grass orb-weaver are extremely rare, as these spiders generally avoid contact and only bite defensively when threatened. The immediate effects of a bite, if it occurs, tend to be mild and not medically significant.

Eastern grass orb-weaver is naturally a non-aggressive creature toward humans, only biting defensively if provoked. This usually occurs when they feel cornered or if a person inadvertently threatens their habitat. Their secretive nature and preference for seclusion are why encounters with humans are unexpected and typically accidental.

To prevent unwanted encounters, it is essential to reduce clutter in homes, seal cracks or crevices, and ensure window screens are intact. Regularly cleaning and dusting will disrupt the habitats of eastern grass orb-weaver and deter them from settling. Keeping vegetation trimmed away from the structure of homes can also be effective.

In the unlikely event of experiencing distress from eastern grass orb-weaver, calmly wash the area with soap and water and apply a cold pack to alleviate any discomfort. It is important to keep the affected area clean to prevent infection. Additionally, monitoring the site for changes is key. Be gentle with the affected area and try to maintain a calm and stress-free environment to aid recovery.