Black and yellow mud dauber has a mild venom that it can deliver through its sting, used primarily for subduing prey rather than defense. Due to the mild nature of the venom, the toxic impact on humans is typically minor, resulting in temporary pain, redness, and swelling. Serious long-term effects or severe complications are rare, with most cases resolving without medical intervention.

Black and yellow mud dauber typically poses a mild threat through its sting, which can cause discomfort among a range of animals, including humans and various domesticated animals such as dogs, cats, and horses. When stung, these animals might experience pain, swelling, and allergic reactions, although it generally isn't severe. The risk is greater for individuals with sensitivities to such stings. Birds might be disturbed by black and yellow mud dauber but tend to suffer less due to their quick avoidance behavior.

Black and yellow mud dauber typically reserve stinging for prey and self-defense. Human stings are rare, occurring primarily when they feel threatened. Only female black and yellow mud dauber possess a stinger, which doubles as an egg-laying organ. A sting generally causes mild pain and swelling for humans, with no significant impact on the black and yellow mud dauber itself.

Black and yellow mud dauber usually uses its sting to immobilize prey and not as a defensive measure. However, close encounters with humans can occur, especially because black and yellow mud daubers may nest in areas frequented by people. When threatened or disturbed, females may sting, causing mild discomfort such as pain, swelling, and redness due to their venom.

To prevent encounters with black and yellow mud dauber, maintain a vigilant effort to keep living spaces clear of nesting sites. Seal cracks and crevices on property structures, as black and yellow mud dauber can utilize these for nests. Regularly inspecting and cleaning gutters, eaves, and outdoor clutter can also deter black and yellow mud dauber from establishing nearby habitats.

If stung by black and yellow mud dauber, cleaning the area with soap and water is a first step to avoid infection. Using a cold compress can help reduce swelling and pain. Distraction and relaxation techniques may alleviate discomfort. During recovery, it's important to avoid disturbing the affected area and keep it clean. Alertness for signs of allergic reactions is essential, albeit rare.