Highveld lesser-thicktail scorpion's venom contains neurotoxins that can affect humans. Juveniles and adults use their sting primarily for defense and predation. Human stings typically result in mild symptoms like local pain and swelling, but severe cases may lead to muscle spasms and hypertension. Long-term effects are rare, yet possible complications include tissue damage at the sting site.

Highveld lesser-thicktail scorpion poses a mild risk via its sting, primarily affecting humans, domestic mammals, and various insects. Its venom is designed to ward off predators and competitors. When stung, humans typically experience localized pain and swelling, while smaller animals like dogs or rats may exhibit more pronounced reactions due to their size. Other insects may be paralyzed or deterred, highlighting the defensive nature of highveld lesser-thicktail scorpion's toxin. Adults of highveld lesser-thicktail scorpion are particularly wary of threats and may sting during this life stage.

Highveld lesser-thicktail scorpion primarily stings in defense when threatened or mishandled by humans. The sting delivers venom that can cause pain and localized swelling in humans, although typically without serious long-term effects. Stinging also serves as a prey capture mechanism

Highveld lesser-thicktail scorpion can cause trouble to humans primarily due to their defensive behavior when they feel threatened. They are armed with venomous stings, which they use to subdue prey or defend against predators, including unintentional threats from humans. Encounters lead to stings that deliver venom, inducing pain and localized swelling in most cases.

Preventative measures against highveld lesser-thicktail scorpion include sealing openings to homes, maintaining clean surroundings to deter their prey, and using physical barriers such as window screens. Keeping outdoor lights off at night to avoid attracting highveld lesser-thicktail scorpion and their prey can also be effective. Regular inspections of dark and undisturbed areas can help in early detection.

If trouble with highveld lesser-thicktail scorpion occurs, promptly cleaning the affected area with soap and water will help prevent infection. Applying a cold compress can reduce pain and swelling. Calm, restful behavior helps to mitigate the spread of venom and symptoms. It's advisable for individuals to stay well-hydrated and observe the affected area for changes. During recovery, careful monitoring and avoiding stressing the affected area should be practiced.