Within the arid and semi-arid regions it inhabits, opistophthalmus macer is recognized for its burrowing behavior, creating underground habitats to escape the harsh surface conditions. This species is nocturnal, emerging at night to hunt a variety of invertebrates with its powerful pedipalps and venomous sting, which, while potent, is not typically lethal to humans. Lifespan can span more than a decade, a testament to its resilience in challenging environments.