Araneus ventricosus typically uses its fangs to inject venom, both for subduing prey and in defense. Although the venom intensity is mild, it can cause discomfort in humans and pets such as dogs and cats, usually resulting in minor symptoms. Small mammals may show more pronounced reactions to a bite. This toxicity is present in both juvenile and adult stages of araneus ventricosus, and while it does not pose a significant threat to larger animals, smaller creatures may suffer more serious consequences.

Araneus ventricosus does not typically exhibit behavior that involves biting humans. These spiders may bite defensively if threatened, but such instances are rare. The bites usually result in mild, localized symptoms due to their non-aggressive nature towards humans.

Araneus ventricosus, by nature, tends not to interact with humans. However, troubles may arise when humans inadvertently threaten their space, such as when gardening, reaching into undisturbed areas, or putting on seldom-used clothing and shoes where araneus ventricosus may hide. These incidental encounters can lead to defensive bites, although commonly with only mild symptoms.

To prevent incidents with araneus ventricosus, keep living and storage areas clean and clutter-free. Regularly inspect and shake out clothing, shoes, and linen that aren't frequently used. Seal gaps in windows and doors, and use screens to deter araneus ventricosus from entering homes. Be mindful when handling stationary objects outdoors.

If trouble with araneus ventricosus occurs, cleaning the area gently with soap and water helps. Applying a cold compress can reduce any mild swelling. Distressed individuals should rest and maintain a clean environment to avoid secondary infections. During recovery, it's important to monitor symptoms and stay in a calm setting, minimizing disturbance to the affected area.