The toxin of spotted wolf spider typically affects other animals when they are bitten. The venom, though mild, is secreted through its fangs and can cause discomfort in humans and domestic animals, such as dogs and cats. Smaller mammals may also be affected. Insects, being more susceptible to the toxin, can suffer more severe consequences. The adults of spotted wolf spider are primarily responsible for producing and delivering the toxin, defending themselves or subduing prey with their venom.

Spotted wolf spider is not known to have a behavior of biting humans. This species typically exhibits biting as a defense mechanism; however, such instances involving humans are extremely rare. If a bite occurs, it is usually due to unintentional provocation, and the consequences are mild due to spotted wolf spider's small size and non-aggressive nature towards humans.

Spotted wolf spider, as a member of the Order Araneae and the Family Lycosidae, doesn't actively seek to bite humans, and such incidents are uncommon. Trouble may arise primarily through accidental contact or provocation, often because spotted wolf spider defends itself when feeling threatened. Due to their small size and general non-aggressiveness, the degree of trouble for humans is typically minimal.

To prevent accidental encounters with spotted wolf spider, maintain cleanliness in areas like basements, garages, and sheds where they may seek shelter. Seal cracks and crevices. Regularly clear out webs and egg sacs. Use screens on windows and doors and keep vegetation around the house trimmed to discourage spotted wolf spider from coming close.

In the rare event of trouble with spotted wolf spider, keeping the affected area clean and refraining from scratching are key. Applying cool compresses can provide relief. Utilize window and door screens to minimize indoor intrusion and consider using LED lights outdoors, as traditional lights may attract more spotted wolf spider. During recovery, avoid disturbing webbed or infested areas to prevent further issues.